Benzonase Nuclease/Binuclease(For the Digestion ofDNA and RNA)

Binuclease is a genetically engineered endonuclease from Serratia marcescens. The  enzyme is produced and purified from eukaryotic yeast cells, without contamination of endotoxin from prokaryotic cells. Functionally, this promiscuous nuclease digests all forms of DNA and RNA, including single stranded, double stranded, linear, circular and supercoiled  DNA and RNA. The enzyme cleaves the phosphodiester bond of nucleic acids, producing 5’ monophosphate terminated oligonucleotides 2-5 bases in length. Binuclease is not a sequence  dependent nuclease, capable of cleaving the phosphodiester bond at all positions among a  nucleic acid chain. This promiscuous endonuclease is applied for removing nucleic acids in biological products. In the industry of vaccine, protein and saccharides pharmaceuticals, this endonuclease is widely used to get rid of nucleic acids, reducing the nucleic acid contaminant  in the vaccines and protein products. This promiscuous nuclease is the ideal choice for  viscosity reduction of cell lysate and cell culture clarification.

CAS No.:	9025-65-4
E.C.:	3.1.30.2
Source:	Serratia marcescens
Cat#:	2604A
Activity:	Solid enzyme ≥ 20 kU/mg lyophilized powder Liquid enzyme ≥ 250 U/μL
Specific activity:	≥ 1000 kU/mg protein
Molecular weight:	27.9 kD
Form:	Solid: white lyophilized powder; Liquid: clear colorless buffered aqueous glycerol solution.
Purity:	≥ 90%; (SDS-PAGE)
Protease:	None detected;
Endotoxin:	None detected (Tachypleus Amebocyte Lysate);
Optimal pH:	8.0 (operational pH 6~10);
Optimal temperature:	37°C (operational 0~42°C);
Activator:	1~10 mM Mg2+
Shelf life:	Solid, 3 years (below 4°C); Liquid, 1 years (-20°C).

Transportation and Storage:

Shipment  of the  liquid form  is below 4°C  or with blue  ice.  Storage  at  -20°C  is recommended.

The solid enzyme can be shipped at room temperature. It is recommended to be stored below 4°C.

Storage buffer: 

20 mM Tris-HCl (pH 8.0), 2 mM MgCl2 , 20 mM NaCl, 50% glycerol.

Dilution buffer:

 20 mM Tris-HCl (pH 8.0), 2 mM MgCl2 , 20 mM NaCl.

 

Unit definition:

According to the protocol from Sigma-Aldrich.

One unit (U) of binuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min under the condition above.

 

1. Removal of nucleic acids (DNA and RNA) in the biological products.

2. Viscosity reduction in cell lysates.