DNA Erasion Kit with DNase I(SpinColumn)-RNA extraction -Beijing Genenode Biotech Co.,Ltd

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DNA Erasion Kit with DNase I(SpinColumn)

Number：R2127

Specifications：50T/200T

Price：350/1260

Package：box

Storage：4℃

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DNA Erasion Kit with DNase I(SpinColumn)

Packing Specification：

R2127-50	DNA Erasion Kit with DNase I(SpinColumn)	50T	CNY350
R2127-200	DNA Erasion Kit with DNase I(SpinColumn)	200T	CNY1260

Product Introduction:
Trace DNA residue cannot be completely avoided during total RNA extraction with any reagent. Due to the unique buffer system and special silica gel adsorption membrane adopted in our RNA extraction products, most DNA contamination can be removed, so DNase I digestion is generally not required. However, for some sensitive downstream experiments that need to remove trace DNA residue, you can purchase our DNase I On-Column Digestion Kit (RNase-free) to directly digest residual DNA on the spin column, and then the pure RNA can be eluted for direct use. This product is compatible with all silica gel membrane spin column-based RNA extraction kits.

Product Features:
1.Simple and fast: The conditions are optimized, and residual DNA on the silica gel membrane can generally be digested and removed within 15 minutes.
2.RNase-free guaranteed: Ensures the integrity of RNA molecules.
3.Wide compatibility: Can be integrated into the on-column digestion process of all silica gel membrane spin column-based RNA extraction kits, eliminating the need to separately remove residual DNA after total RNA extraction.

Application Scope:
Suitable for sensitive downstream experiments that require the removal of trace DNA residue. It directly digests residual DNA on the spin column, and the pure RNA can be eluted for direct use.

Experimental Procedures:
1.Perform the normal RNA extraction steps until the lysis mixture is completely centrifuged through the column (RNA and residual DNA are adsorbed onto the silica gel membrane of the spin column). Before adding Deproteinization Buffer RW1, follow the steps below:

2.Take a new 1.5 mL centrifuge tube, add 45 μL of DNase I Buffer and 5 μL of RNase-free DNase I, and gently pipette to mix well to prepare the DNase I working solution (scale up the volume proportionally when processing multiple columns). Set aside on ice.
Note: If residual DNA is excessive leading to incomplete digestion, increase the enzyme amount proportionally to improve digestion efficiency (e.g., 90 μL of DNase I Buffer and 10 μL of RNase-free DNase I).

3.Add 350 μL of Deproteinization Buffer RW1 to the SpinRA column, centrifuge at 12,000 rpm for 30 seconds, discard the waste liquid, and place the spin column back into the collection tube.

4.Add 50 μL of the DNase I working solution to the center of the SpinRA column. Incubate at room temperature (20-30°C) for 15 minutes. Note: Add the working solution directly to the center of the membrane; do not let it drip onto the O-ring or the inner wall of the centrifuge column.

5.Add 350 μL of Deproteinization Buffer RW1 to the SpinRA column, centrifuge at 12,000 rpm for 30-60 seconds, discard the waste liquid, and place the spin column back into the collection tube.
Proceed to the subsequent steps such as washing with Wash Buffer RW. For kits from other companies, proceed to the final washing step with the last wash buffer and other subsequent steps.

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