RNase Inhibitor(Rnasin)-RNA extraction -Beijing Genenode Biotech Co.,Ltd

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RNase Inhibitor(Rnasin)

Number：R2130

Specifications：2KU/10KU

Price：240/1100

Package：box

Storage：4℃

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RNase Inhibitor(Rnasin)

Packing Specification：

R2130-2	RNase Inhibitor(Rnasin)	2KU	CNY240
R2130-10	RNase Inhibitor(Rnasin)	10KU	CNY1100

Product Introduction:
This product is a recombinant protein expressed in Escherichia coli. It forms a 1:1 complex with RNase A and exhibits highly non-competitive inhibition of RNases (Ki = 3×10⁻¹⁰ M). The reaction is reversible: the complex can be dissociated by urea and thiol-containing reagents, resulting in the reactivation of RNase and the irreversible inactivation of the inhibitor. As a proteinaceous inhibitor, it differs from other competitive inhibitors (nucleic acid-based, inorganic phosphate-based) and can be easily removed from the reaction system by phenol treatment.

Activity Definition:

1.One activity unit (U) is defined as the amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A.

2.First-Strand cDNA Synthesis (Taking 20 μL Reaction System as Example)
1) Add the Following Components

Components	Volume
Total RNA/mRNA	50 ng-5 μg/5-500 ng
Oligo(dT)18(0.5 μg /μL)or Random Primer(0.1 μg/μL) or GSP(Gene Specific Primer)	1 μL
	1 μL
	2 pmol
dNTP Mixture (10 mM each)	1 μL
5× RT Buffer	4 μL
RNase Inhibitor(40 units/μL)	0.5-1 μL
TUREscript Hˉ RTase	1 μL
RNase free H2O to final volume	20 μL

2) Incubation Conditions
3) Mix gently.
If using Oligo (dT)₁₈ or Gene-Specific Primer (GSP), incubate at 42°C for 50 minutes.
If using Random Primer, incubate at 25°C for 10 minutes, then at 42°C for 50 minutes.
Heat at 70°C for 5 minutes to inactivate TUREscript H⁻ RTase.

3.RT-PCR
The cDNA obtained by reverse transcription can be directly used for PCR reactions or stored at -20°C.

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